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KMID : 1141020210210010011
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Journal of Applied Oriental Medicine
2021 Volume.21 No. 1 p.11 ~ p.42
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Chondroprotective Effects of Salviae Plebeiae Herba in a Rat Model of Osteoarthritis
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Lee Soo-Hwa
Seo Bu-Il
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Abstract
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Salviae Plebeiae Herba (SP) contains a numberous chemical components and reported to exert various pharmacological activities such as antioxidant, anti-inflammatory, antimicrobial, analgesic, hypoglycemic, antiviral, anti-tumour, and hepatoprotective effects. Even though it has these various effects, its scientific proof remains uncertain the effect and underlying mechanism about joint damage. Accordingly, the present study was designed to evaluate the therapeutic effects and possible underlying mechanism of SP on osteoarthritis. OA models were induced via intra-joint injection of monosodium iodoacetate (MIA) 50 ㎕ with 80 ㎎/㎖ in rats. Excluding the normal group, OA-induced mice were divided into 4 groups (control, INDO, SPL, SPH). The drug concentrations were indomethacin 5 ㎎/㎏, SP 500 ㎎/㎏, and SP 1000 ㎎/㎏, and were administered orally once a day for 2 weeks. After drug supplementation, the effects of SP were measured with serum analysis, western blotting, and histopathological staining. SP administration improved hind limb overload and it led to relieve pain. Moreover, it suppressed oxidative related factors. The NF-κB inactivation though the inhibition of IκBα phosphorlyation significantly decreased both inflammatory mediators such as COX-2 and iNOS and cytokines such as TNF-α, IL-1β, and IL-6. Whereas, anti-inflammatory cytokines like IL-4 and IL-10 were shown to increase. In addition, SP administration exerted a protective effect on ECM by reducing the expression of collagen degradation factors including MMP-1, -3, -13 and by increasing TIMP-1. Taken together, SP may possess the effect of delaying the progression of osteoarthritis and protecting cartilage. Accordingly, the present study may provide a scientific basis the chondroprotective effect of SP on MIA induced osteoarthritis.
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KEYWORD
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MIA, osteoarthritis, Salviae Plebeiae Herba, anti-inflammatory
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